(-)-Butin

SKU: orb1297436

Description

(-)-Butin is a natural flavonoid with potent antioxidant properties. It activates the Nrf2 pathway via PI3K/Akt signaling to upregulate MnSOD, thereby protecting cells from H2O2-induced apoptosis and mitochondrial dysfunction in vitro. This mechanism supports its research in models of oxidative stress-related diseases.

Research Area

Immunology & Inflammation, Signal Transduction

Images & Validation

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Key Properties

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CAS Number	492-14-8
MW	272.25
Purity	99.96% (May vary between batches)
Formula	C₁₅H₁₂O₅
SMILES	Oc1ccc2C(=O)C[C@H](Oc2c1)c1ccc(O)c(O)c1
Target	Nrf2,Akt,PI3K
Solubility	DMSO:50 mg/mL (183.65 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:4 mg/mL (14.69 mM)

Bioactivity

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In Vitro

The antioxidant function of manganese superoxide dismutase (Mn SOD) is important in preventing oxidative stress. While exposure to H2O2 reduced the expression of Mn SOD in Chinese hamster lung fibroblast (V79-4), the addition of Butin restored Mn SOD expression at both the mRNA and protein levels, resulting in increased Mn SOD activity. The transcription factor NF-E2-related factor 2 (Nrf2) regulates Mn SOD gene expression by binding to the antioxidant responsive element (ARE). Butin enhanced the nuclear translocation and ARE-binding activity of Nrf2, which was decreased by H2O2. The siRNA-mediated knockdown of Nrf2 attenuated Butin-induced Mn SOD expression and activity. Further, phosphatidylinositol 3-kinase (PI3K)/protein kinase B (PKB, Akt) contributed to the ARE-driven Mn SOD expression. Butin activated PI3K/Akt and exposure to either LY294002 (a PI3K inhibitor), Akt inhibitor IV (an Akt-specific inhibitor), or Akt siRNA suppressed the Butin-induced activation of Nrf2, resulting in decreased Mn SOD expression and activity. Finally, the cytoprotective effect of Butin against H2O2-induced cell damage was suppressed by the siRNA-mediated knockdown of Mn SOD

Storage & Handling

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Storage	Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice/Shipping at ambient temperature.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Butin, Akt, ( ) Butin, ()Butin, inhibit, Nrf2, Inhibitor, PI3K

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Compound Identifiers

PubChem CID

92775 ↗

Key Properties

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No computed properties available.

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(-)-Butin (orb1297436)

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Purity:	99.91%
CAS Number:	21913-99-5
MW:	272.25
Formula:	C₁₅H₁₂O₅

CAS Number:	74628-42-5
MW:	272.25
Formula:	C₁₅H₁₂O₅

Purity:	>98% (HPLC)
CAS Number:	492-14-8
MW:	272.25
Formula:	C₁₅H₁₂O₅

Purity:	>98% (HPLC)
CAS Number:	21913-99-5
MW:	272.25
Formula:	C₁₅H₁₂O₅

(-)-Butin

Description

Research Area

Images & Validation

Key Properties

Bioactivity

Storage & Handling

Alternative Names

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