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DDX6 Antibody
Description
Images & Validation
−| Tested Applications | IF, IHC, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 400 to the C-terminus of human DDX6 (NP_001244120.1). |
| Target | DDX6 |
| Molecular Weight | Observed: 54kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
| Concentration | batch dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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Western blot analysis of extracts of various cell lines, using DDX6 antibody (orb1259076) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Immunohistochemistry of paraffin-embedded rat brain using DDX6 antibody (orb1259076) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human lung cancer using DDX6 antibody (orb1259076) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human colon carcinoma using DDX6 antibody (orb1259076) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human mammary cancer using DDX6 antibody (orb1259076) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human placenta using DDX6 antibody (orb1259076) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse kidney using DDX6 antibody (orb1259076) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of L929 cells using DDX6 Polyclonal Antibody (orb1259076) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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DDX6 Antibody (orb1259076)
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