Donkey IgG (H&L) Antibody Alkaline Phosphatase Conjugated

SKU: orb348002

Description

Donkey IgG (H&L) antibody (Alkaline Phosphatase)

Images & Validation

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Tested Applications	ELISA, IHC, WB
Dilution range	ELISA: 1:15,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
Reactivity	Donkey
Application Notes	Anti-Donkey IgG Alkaline Phosphatase Antibody has been tested by ELISA and is assayed against 1.0 ug of Donkey IgG in a standard capture ELISA using pNPP (p-nitrophenyl phosphate ) as a substrate for 30 minutes at room temperature. A working dilution of 1:3,000 to 1:15,000 of the reconstitution concentration is suggested for this product.

Key Properties

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Antibody Type	Secondary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Donkey IgG whole molecule
Purity	This product was prepared from monospecific antiserum by immunoaffinity chromatography using Donkey IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Donkey IgG and Donkey Serum.
Conjugation	AP

Storage & Handling

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Storage	Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Form/Appearance	Liquid (sterile filtered)
Buffer/Preservatives	0.1% (w/v) Sodium Azide
Concentration	1.0 mg/mL
Disclaimer	For research use only

Alternative Names

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rabbit anti-Donkey IgG Antibody alkaline phosphatase Conjugation, rabbit anti-Donkey IgG alk phos Conjugated Antibody

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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