F(ab')2 Goat IgG (H&L) Antibody Phycoerythrin conjugated

SKU: orb348113

Description

F(ab')2 Goat IgG (H&L) antibody (RPE)

Images & Validation

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Tested Applications	FC, IF
Dilution range	FC: 1:100 - 1:250, IF: 1:100 - 1:250
Reactivity	Goat
Application Notes	This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Key Properties

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Antibody Type	Secondary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG F(ab')2
Immunogen	Goat IgG whole molecule
Purity	F(ab')2 fragment antibody Anti-Goat IgG was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by pepsin digestion and chromatographic separation. Coupling to R-PE was followed by size exclusion chromatography to purify conjugate from unreacted R-PE and antibody. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Rabbit Serum, Goat IgG and Goat Serum. No reaction was observed against anti-Pepsin or anti-Rabbit IgG F(c).
Conjugation	RPE

Storage & Handling

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Storage	Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent). This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. Do not freeze after reconstitution. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis.
Form/Appearance	Lyophilized
Buffer/Preservatives	Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration	0.5 mg/mL
Disclaimer	For research use only

Alternative Names

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Rabbit F(ab')2 Anti-Goat IgG Antibody Phycoerythrin Conjugated, Rabbit Fab2 Anti-Goat IgG Antibody PE Conjugation

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Protocol Information

Flow Cytometry

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Immunofluorescence

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