F(ab')2 Rabbit IgG (H&L) Antibody Pre-Adsorbed

SKU: orb348302

Description

F(ab')2 Rabbit IgG (H&L) antibody

Images & Validation

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Item 1 of 3

Tested Applications	ELISA, IHC, WB
Dilution range	ELISA: 1:20,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
Reactivity	Rabbit
Application Notes	F(ab')2 Anti-Rabbit IgG Antibody has been tested by SDS-PAGE and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 µg of antibody. Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications. Read more...

Key Properties

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Antibody Type	Secondary Antibody
Host	Goat
Clonality	Polyclonal
Isotype	IgG F(ab')2
Immunogen	Rabbit IgG whole molecule
Purity	This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins.
Conjugation	Unconjugated

Storage & Handling

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Storage	Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
Form/Appearance	Liquid (sterile filtered)
Buffer/Preservatives	0.01% (w/v) Sodium Azide
Concentration	1.0 mg/mL
Disclaimer	For research use only

Alternative Names

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Goat F(ab')2 Anti-Rabbit IgG Antibody Pre-Adsorbed, Goat Fab2 Anti-Rabbit IgG Antibody

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

F(ab')2 Rabbit IgG (H&L) Antibody Pre-Adsorbed

KIF20B shRNA efficiency. (B) The shRNA efficiency measured by western blotting. Primary antibodies used rabbit anti-KIF20B antibody [1:100] and mouse anti-β-actin [1:500] with goat anti-rabbit [1:10000] and goat anti-mouse Fc secondary antibody [1:10000]. *P < 0.05 vs. control. KIF, kinesin family member; shRNA, short hairpin RNA.

SDS-PAGE results of Goat F(ab')2 Anti-Rabbit IgG (H&L) Antibody. Lane 1: reduced F(ab')2 anti-Mouse. Lane 2: reduced F(ab')2 anti-Rabbit. Lane 3: Opal Prestained Ladder. Lane 4: non-reduced F(ab')2 anti-Mouse. Lane 5: non-reduced F(ab')2 anti-Rabbit. Load: 1.0ug. 4-20% Lonza SDS-PAGE; Coomassie Stained.

Western Blot results using Goat F(ab')2 Anti-Rabbit IgG. Effects of RAB38 silencing on the proliferation ability of PANC-1 and BxPC-3 cells. The expression levels of the proliferation-associated proteins (C) Ki67 and (D) PCNA in PANC-1 and BxPC-3 cells were detected by western blot analysis. Data are reported as the mean ± standard deviation (n = 3). *P < 0.05 vs. control group. shRNA, small hairpin RNA; RAB38, Ras-related GTP-binding protein 38; OD, optical density; PCNA, proliferating cell nuclear antigen.

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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