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AIF1/IBA1 (isoform 3) Antibody

SKU: orb19198

Description

Goat polyclonal antibody to Interferon gamma

Research Area

Musculoskeletal & Connective Tissue Research

Images & Validation

Tested ApplicationsELISA, FC, IF, IHC
Dilution RangeELISA: 1:32000, WB: 0.5-1.5 μg/ml, FACS: 10ug/ml, IF/ICC: 10μg/ml
ReactivityHuman
Predicted ReactivityCanine, Mouse
Application Notes
IHC: Paraffin embedded Human Lung and Brain (Cortex). Recommended concentration: 5-6µg/ml.. Notes: Immunofluorescence: Strong expression of the protein seen in the cytoplasm and plasma membrane of U937, Caco-2 and U251 cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of K562 cells

Key Properties

ClonalityPolyclonal
TargetAIF1 / IBA1 (isoform 3)
Protein SequenceNKQFLDDPKYSSDED
Molecular Weight16.7
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

AIF1, IBA1, allograft inflammatory factor 1 , DASS-82G15.2, AIF-1, IRT-1, Em:AF129756.17, interferon gamma responsive transcript, ionized calcium-binding adapter molecule

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NCBI Gene Details

No NCBI Gene data available

Protocol Information

IHC
Immunohistochemistry
View Protocol
FC
Flow Cytometry
View Protocol
IF
Immunofluorescence
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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100 μg
$ 560.00
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Free Secondary Antibody (20 ul)0/0

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