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SHH Antibody

SKU: orb527297

Description

Goat polyclonal antibody to SHH

Images & Validation

Tested ApplicationsELISA, FC, IF, WB
Dilution rangeP-ELISA: 1: 32000
ReactivityHuman, Mouse
Predicted ReactivityCanine, Rat
Application Notes
WB: Approx 48kDa band observed in Human Liver lysates (calculated MW of 49.6kDa according to NP_000184.1 Recommended concentration: 1-3µg/ml. Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on Rat Liver for this particular . Notes: Immunofluorescence: Strong expression of the protein seen in the membranes of HeLa, U251 and NIH3T3 cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concen

Key Properties

HostGoat
ClonalityPolyclonal
TargetSHH
Molecular Weight49.6
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
DisclaimerFor research use only

Alternative Names

SHH, sonic hedgehog homolog (Drosophila), HGNC:10848, HHG1, HLP3, HPE3, SMMCI , sonic hedgehog

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SHH Antibody

WB analysis of Human Liver lysate using SHH antibody

SHH Antibody

Immunofluorescence analysis of HeLa cells using SHH antibody

SHH Antibody

Immunofluorescence analysis of NIH3T3 cells using SHH antibody

SHH Antibody

Flow cytometric analysis of HeLa cells using FOXC2 antibody

SHH Antibody

Immunofluorescence analysis of U251 cells using SHH antibody

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
FC
Flow Cytometry
View Protocol
IF
Immunofluorescence
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

SHH Antibody (orb527297)

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Available Sizes

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100 μg
$ 530.00
DispatchUsually dispatched within 5-10 working days
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