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IL-1 beta Recombinant Protein
Description
Research Area
Images & Validation
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Key Properties
−| Source | E. coli |
|---|---|
| Biological Activity | The ED50 was determined by the dose - dependent stimulation of thymidine uptake by murine D10S cells is ≤ 1.0 pg/mL, corresponding to a specific activity of ≥ 1 x 109 units/mg. |
| Target | IL1B |
| Protein Sequence | APVRSLNCTL RDSQQKSLVM SGPYELKALH LQGQDMEQQV VFSMSFVQGE ESNDKIPVAL GLKEKNLYLS CVLKDDKPTL QLESVDPKNY PKKKMEKRFV FNKIEINNKL EFESAQFPNW YISTSQAENM PVFLGGTKGG QDITDFTMQF VSS |
| Purity | Greater than 98% by SDS-PAGE gel and HPLC analyses.Endotoxin level is less than 0.1 ng per μg (1EU/μg). |
Storage & Handling
−| Storage | The lyophilized IL-1 beta recombinant protein is stable for at least 2 years from date of receipt at -20°C. Reconstituted IL-1 beta is stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. As with any protein, exposing IL-1 beta recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature. |
|---|---|
| Form/Appearance | Lyophilized |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Recombinant Human IL1B Protein, hFc Tag [orb1173751]
Unconjugated
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 43.5 kDa after removal of the signal peptide.The apparent molecular mass of IL1B-hFc is approximately 35-55 kDa due to glycosylation.
Mammalian
10 μg, 100 μg, 50 μgRecombinant Human Receptor-interacting serine/threonine-protein kinase 2 (RIPK2) [orb1785613]
Greater than 90% as determined by SDS-PAGE.
62.0 kDa
E.coli
1 mg, 100 μg, 20 μg

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Quick Database Links
UniProt Details
−NCBI Reference Sequences
−| Protein | NP_000567.1 |
|---|
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Protocol Information
IL-1 beta Recombinant Protein (orb1230861)
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