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KI-67/MKI67 Antibody
SKU: orb1294315
Description
Images & Validation
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| Tested Applications | IF, IHC, WB |
|---|---|
| Dilution range | Western Blot 1:500–1:1000 Immunofluorescence 1:200–1:500 Immunohistochemistry (Paraffin) 1:100–1:200 |
| Reactivity | Human |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | Rabbit-IgG |
| Immunogen | Synthetic peptide / encompassing a sequence within the C-terminus region. |
| Target | KI-67 / MKI67 |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Disclaimer | For research use only |
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anti-Ki-67 antibody - at 1/500 dilution, Lysates at 50 µg per lane, This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked with 3% BSA an hour before being incubated with orb1294315 overnight at 4°C.
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294315 anti-Ki-67 antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Ki-67 with orb1294315 at 1/200.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Ki-67 with orb1294315 at 1/200.
Quick Database Links
Gene Symbol
KI-67 / MKI67
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
KI-67/MKI67 Antibody (orb1294315)
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