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MKI67 Antibody
SKU: orb1258827
Featured
Description
Images & Validation
−Item 1 of 3
| Tested Applications | IF, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant protein of human Ki67 |
| Target | MKI67 |
| Molecular Weight | Observed: 358kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Liquid |
| Concentration | batch dependent |
| Disclaimer | For research use only |
Alternative Names
−monoclonal Ki-67, Proliferation-related Ki-67 antigen, Ki-67, KIA, HGNC:7107, antigen identified by monoclonal Ki-67, MKI67, MIB-1
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Western blot analysis of extracts of NIH/3T3 cells, using Ki67 antibody (orb1258827) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunofluorescence analysis of C6 cells using Ki67 antibody (orb1258827) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using Ki67 antibody (orb1258827) at dilution of 1:100. Blue: DAPI for nuclear staining.
Quick Database Links
Gene Symbol
MKI67
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
MKI67 Antibody (orb1258827)
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