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Validated
Description
Research Area
Images & Validation
−| Tested Applications | IHC, KO/KD Validated, WB |
|---|---|
| Dilution Range | IHC 1:150 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rat |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone No. | BQVK5D8 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1160-1493 of human MKI67 (NP_002408) produced in E.coli. |
| Target | Ki67 |
| Purification | Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G) |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS (pH 7.3) containing 1% rAlbumin, 50% glycerol and 0.02% sodium azide. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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Western blot analysis of Equivalent amounts of cell lysates (10 ug per lane) of wild-type Hela cells (WT) and MKI67-Knockout Hela cells (KO) were separated by SDS-PAGE and immunoblotted using Ki67 (MKI67) antibody
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KI67 Antibody (orb1318782)
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