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PCNA Mouse Monoclonal Antibody
SKU: orb629725
Featured
Description
Research Area
Cancer, DNA Damage, Epigenetics, Infectious Diseases, Neuroscience
Images & Validation
−Item 1 of 4
| Tested Applications | ELISA, FC, IHC, IP, WB |
|---|---|
| Dilution Range | WB: 1:5000-1:50000; IP: 1:200-1:1000; IHC: 1:500-1:2000; IF: 1:50-1:500 |
| Reactivity | Human, Mouse, Rabbit, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Immunogen | proliferating cell nuclear antigen |
| Target | PCNA |
| Purity | ≥95% as determined by SDS-PAGE |
| Purification | Protein A+G purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | liquid |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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HEK-293 cells were subjected to SDS PAGE followed by western blot with orb629725 (PCNA Antibody) at dilution of 1:10000.
Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using orb629725 (PCNA antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG (H+L).
IP Result of anti-PCNA (IP: orb629725, 4 ug; Detection: orb629725 1:300) with HepG2 cells lysate 3000 ug.
Immunohistochemistry of paraffin-embedded human breast cancer using orb629725 (PCNA antibody) at dilution of 1:800.
Quick Database Links
Gene Symbol
PCNA
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
FC
Flow Cytometry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
IP
Immunoprecipitation
PCNA Mouse Monoclonal Antibody (orb629725)
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