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Physalin A

SKU: orb1681906

Description

Physalin A, a natural compound from Physalis alkekengi, exhibits anti-inflammatory and anti-fibrotic activity by modulating the Nrf2 pathway via ERK/p38 and inhibiting NF-κB signaling. It also reduces mammosphere formation by downregulating GLI1 and YAP1, making it a useful research tool for studying fibrosis, inflammation, and cancer stem cells in vitro.

Research Area

Cardiovascular Research, Cell Biology, Epigenetics & Chromatin, Signal Transduction, Stem Cell & Developmental Biology

Images & Validation

Key Properties

CAS Number23027-91-0
MW526.53
Purity99.71%
FormulaC28H30O10
SMILESC[C@@]12[C@]34[C@]([C@]5(C)C[C@]1(OC(=O)C5=C)[H])(C(=O)[C@](O)(O3)[C@]6([C@](CC[C@]4(O)C(=O)O2)([C@]7(C)C(=C[C@H]6O)CC=CC7=O)[H])[H])[H]
TargetApoptosis,Autophagy,JAK,mTOR,PI3K,Akt,STAT,p38 MAPK,NF-κB
SolubilityDMSO:55.34 mg/mL (105.1 mM)

Bioactivity

Target IC50
HT1080 cells:10.7 μM
In Vivo
After anesthetization with 1% pentobarbital (100 µl/10 g body weight, intraperitoneal administration), 24 mice were randomly selected and grouped (the DMM group and the DMM + Physalin A group) for destabilized medial meniscus (DMM) surgery. Three groups were administered intra-articular injections with different treatments, twice per week. The sham and DMM groups received 10 μl of the vehicle (30% PEG300, 5% DMSO, and ddH2O), whereas the DMM + Physalin A group received 10 μl of Physalin A (1 mg/kg body weight). Physalin A decreasing MMP13 production and increasing collagen II and aggrecan production in the in vivo mouse OA model. The OARSI score also indicated that injecting Physalin A into the knee joint cavity could ameliorate the progression of OA in the mouse model.
In Vitro
To detect the growth inhibition effects of Physalin A on different human tumor cell lines (HT1080 fibrosarcoma, A375-S2 melanoma, HepG2 hepatoma, HeLa cervical carcinoma, A549 alveolar basal epithelial, U937 histocytic lymphoma, HCT116 colon cancer, A431 epidermoid carcinoma, MCF7 breast cancer, and HL60 promyelocytic leukemia cells), the cells were cultured with 0, 10, 20, 40, and 80 μM Physalin A for 24 h. HT1080 and A375-S2 cells were the most sensitive tumor cell lines among those used. Physalin A inhibited HT1080 cell growth in a time- and dose-dependent manner with an IC50 value (at 24 h) of 10.7 μM.

Storage & Handling

Storagekeep away from moisture,keep away from direct sunlight,store at low temperature | store at -20°C | Shipping with blue ice/Shipping at ambient temperature.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

ERK, p53, NF-κB, JNK, JAK, Autophagy, Apoptosis, Androgen Receptor, Bcl-2, p38 MAPK, Physalin A, STAT, ROS

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Key Properties

No computed properties available.

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Physalin A (orb1681906)

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% DMSO +
%+
% Tween 80 +
%

Available Sizes

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1 mg
$ 340.00
5 mg
$ 810.00
10 mg
$ 1,260.00
1 ml x 10 mM (in DMSO)
$ 1,290.00
25 mg
$ 1,860.00
50 mg
$ 2,490.00
DispatchUsually dispatched within 5-10 working days
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