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Recombinant Human CD3E Protein
SKU: orb2993920
Description
Images & Validation
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Key Properties
−| Expression System | Human Cells |
|---|---|
| Biological Origin | Human |
| Biological Activity | ELISA: Immobilized Anti-Human/Monkey CD3E mAb (Cat#orb2993159) at 2μg/ml (100 μl/well) can bind Human CD3E-mFc (Cat#orb2993920).The ED50 of Human CD3E-mFc (Cat#orb2993920) is 28.99 ng/ml.. BLI: Loaded Anti-Human/Monkey CD3E mAb-Fc(Cat#orb2993159) on AHC Biosensor, can bind Human CD3E-mFc(Cat#orb2993920) with an affinity constant of -3 nM as determined in BLI assay. |
| Tag | C-mFc |
| Molecular Weight | Predicted: 28.5 KDa. Observed: 32-40 KDa, reducing conditions |
| Expression Region | Gln22-Thr48 |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.2 μm filtered solution of PBS, pH 7.4. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−T-Cell Surface Glycoprotein CD3 Epsilon Chain; T-Cell Surface Antigen T3/Leu-4 Epsilon Chain; CD3e; CD3E; T3E
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Recombinant Human CD3E Protein (orb2993920)
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