SARS-CoV-2 (COVID-19) Spike S2 Antibody

SKU: orb1239031

Description

Images & Validation

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Item 1 of 2

Tested Applications	ELISA, WB
Reactivity	Virus

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Anti-SARS-CoV-2 (COVID-19) Spike antibody (orb1239031) was raised against a peptide corresponding to 16 amino acids near the center terminus of SARS-CoV-2 (COVID-19) Spike glycoprotein. The immunogen is located within 900-950 amino acids of SARS-CoV-2 (COVID-19) Spike protein.
Target	S
Purification	SARS-CoV-2 (COVID-19) Spike Antibody is affinity chromatography purified via peptide column.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	SARS-CoV-2 (COVID-19) Spike Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration	1 mg/mL
Disclaimer	For research use only

Alternative Names

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SARS-CoV-2 (COVID-19) Spike Antibody: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Surface Glycoprotein, Spike protein

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Western Blot Validation with SARS-CoV-2 (COVID-19) Spike S2 ECD Recombinant Protein. Loading: 30 ng per lane of SARS-CoV-2 (COVID-19) Spike S2 ECD recombinant protein, orb1231609. Antibodies: SARS-CoV-2 (COVID-19) Spike S2, orb1239031, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 1 µg/mL and Lane 2: 2 µg/mL.

ELISA Validation. Antibodies: SARS-CoV-2 (COVID-19) Spike S2 Antibody, orb1239079. A direct ELISA was performed using immunogen peptide (orb1231697) as coating antigen and the anti-SARS-CoV-2 (COVID-19) Spike S2 antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL.

Quick Database Links

Gene Symbol

UniProt

P0DTC2

RefSeq:QHD43416

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

RefSeq	QHD43416

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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