Recombinant Spectrin beta III Antibody / SPTBN2

SKU: orb2642732

Description

Spectrin is an actin binding protein that is a major component of the plasma membrane skeleton. Spectrins function as membrane organizers and stabilizers by forming dimers, tetramers and higher polymers. Vertebrate spectrins have two alpha-subunits (alpha-I/alpha-II), four beta-subunits (beta-I-beta-IV) and a beta-H subunit creating diversity and specialization of function. Spectrin and spectrin are present in erythrocytes, whereas spectrin II (also designated fodrin ) and spectrin I (also designated fodrin ) are present in other somatic cells. The spectrin tetramers in erythrocytes act as barriers to lateral diffusion, but spectrin dimers seem to lack this function. Spectrin III is highly homologous to both spectrin I and spectrin II. Spectrin III is highly expressed in brain, kidney, pancreas and liver, and at lower levels in lung and placenta.

Images & Validation

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Tested Applications	ELISA, IF, IHC-P, WB
Dilution Range	ELISA: 2-4ug/ml (order BSA/azide-free format),Western blot: 1-2ug/ml,Flow cytometry: 0.5-1ug/10^6 cells Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Reactivity	Human
Application Notes	The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the recombinant Spectrin beta III antibody to be titered up or down for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. Read more...

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	Rabbit IgG, kappa
Clone No.	SPTBN2/3142R
Immunogen	Human recombinant partial protein corresponding to amino acids 356-475 was used as the immunogen for this recombinant Spectrin beta III antibody.
Purification	Protein A affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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Recombinant Spectrin beta III Antibody / SPTBN2

Flow cytometry staining of human HeLa cells with Spectrin beta III antibody; Red=isotype control, Blue= Spectrin beta III antibody.

Western blot testing of human HeLa, PC-3 and brain lysate with recombinant Spectrin beta III antibody (clone SPTBN2/3142R). Predicted molecular weight ~246 kDa.

Immunofluorescent staining of human HeLa cells with recombinant Spectrin beta III antibody (green, clone SPTBN2/3142R) and Reddot nuclear stain (red).

SDS-PAGE analysis of purified, BSA-free recombinant Spectrin beta III antibody (clone SPTBN2/3142R) as confirmation of integrity and purity.

IHC testing of FFPE human pancreas with recombinant Spectrin beta III antibody (clone SPTBN2/3142R). Required HIER: boil tissue sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.

Quick Database Links

UniProt

O15020

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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Immunofluorescence

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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