Recombinant Spectrin beta III Antibody / SPTBN2

SKU: orb606846

Description

Spectrin is an actin binding protein that is a major component of the plasma membrane skeleton. Spectrins function as membrane organizers and stabilizers by forming dimers, tetramers and higher polymers. Vertebrate spectrins have two alpha-subunits (alpha-I/alpha-II), four beta-subunits (beta-I-beta-IV) and a beta-H subunit creating diversity and specialization of function. Spectrin and spectrin are present in erythrocytes, whereas spectrin II (also designated fodrin ) and spectrin I (also designated fodrin ) are present in other somatic cells. The spectrin tetramers in erythrocytes act as barriers to lateral diffusion, but spectrin dimers seem to lack this function. Spectrin III is highly homologous to both spectrin I and spectrin II. Spectrin III is highly expressed in brain, kidney, pancreas and liver, and at lower levels in lung and placenta.

Research Area

Epigenetics, Neuroscience

Images & Validation

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Tested Applications	ELISA, FACS, IHC-P, WB
Dilution Range	Flow cytometry: 1-2ug/10^6 cells,ELISA: 2-4ug/ml (order BSA/azide-free format),Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Reactivity	Human
Application Notes	The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the recombinant Spectrin beta III antibody to be titered up or down for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. Read more...

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	Rabbit IgG, kappa
Clone No.	SPTBN2/2894R
Immunogen	Human recombinant partial protein corresponding to amino acids 356-475 was used as the immunogen for this recombinant Spectrin beta III antibody.
Purification	Protein A affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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Recombinant Spectrin beta III Antibody / SPTBN2

IHC testing of FFPE human pancreatic cancer with recombinant Spectrin beta III antibody (clone SPTBN2/2894R). Required HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 min.

Western blot testing of human PANC-1 and HeLa cell lysate with recombinant Spectrin beta III antibody (clone SPTBN2/2894R). Predicted molecular weight ~246 kDa.

FACS testing of fixed and permeabilized human HeLa cells with recombinant Spectrin beta III antibody (blue) and isotype control (red).

SDS-PAGE analysis of purified, BSA-free recombinant Spectrin beta III antibody (clone SPTBN2/2894R) as confirmation of integrity and purity.

Quick Database Links

UniProt

O15020

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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FACS

Fluorescence-Activated Cell Sorting (FC, Flow cytometry)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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