STAT3 Rabbit Polyclonal Antibody

SKU: orb783385

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Description

Images & Validation

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Item 1 of 3

Tested Applications	WB
Dilution range	WB=1:1000-2000
Reactivity	Human
Predicted Reactivity	Mouse, Rat

Related Conjugates & Formulations

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Biotin

HRP

IRDye800CW

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Recombinant human STAT3 (640-770aa)
Target	STAT3
Molecular Weight	88 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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1110034C02Rik; Acute Phase Response Factor; APRF; AW109958; DNA binding protein APRF; FLJ20882; HIES; MGC128731; MGC16063; MGC93551; Signal transducer and activator of transcription 3(acute-phase response factor); Signal transducer and activator of transcription 3; Signal Transductor and Activator of Transcription 3; STAT 3; Transcription factor; STAT3_HUMAN.

Phospho-STAT3 (Tyr705) Rabbit Polyclonal Antibody [orb608174]

Applications:	IF, IHC-Fr, IHC-P
Predicted Reactivity:	Bovine, Canine, Gallus, Guinea pig, Porcine, Rabbit, Rat, Sheep
Reactivity:	Human, Mouse, Rat
Species/Host:	Rabbit
Clonality:	Polyclonal
Conjugation:	Unconjugated

Sizes Available: 100 μl, 200 μl, 50 μl

Item 1 of 6
STAT3 Rabbit Polyclonal Antibody [orb592766]
Applications:
IHC, WB
Predicted Reactivity:
Bovine, Canine, Equine, Guinea pig, Mouse, Porcine, Rabbit, Rat, Sheep
Reactivity:
Human
Species/Host:
Rabbit
Clonality:
Polyclonal
Conjugation:
Unconjugated
Sizes Available: 100 μl
Item 1 of 7
Stat3 Polyclonal Antibody [orb1412088]
Applications:
IF, IHC-P, WB
Reactivity:
Human, Mouse, Rat
Species/Host:
Rabbit
Clonality:
Polyclonal
Conjugation:
Unconjugated
Sizes Available: 100 μl

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Paraformaldehyde-fixed, paraffin embedded (human myocardium), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (STAT3) Polyclonal Antibody, Unconjugated (orb783385) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Heart (Mouse) Tissue Lysate at 40 ug, Lane 2: Kidney (Mouse) Tissue Lysate at 40 ug, Lane 3: Cerebrum (Mouse) Tissue Lysate at 40 ug, Lane 4: Heart (Rat) Tissue Lysate at 40 ug, Lane 5: Kidney (Rat) Tissue Lysate at 40 ug, Lane 6: Cerebrum (Rat) Tissue Lysate at 40 ug, Primary: Anti-STAT3 (orb783385) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 88 kD, Observed band size: 84 kD.

Sample: Lane 1: Human Hela Cell (Control) Lysate, Lane 2: STAT3 knockout (KO) HeLa Cell Lysate, Primary: Anti-STAT3 (orb783385) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 88 kD, Observed band size: 72 kD.

Quick Database Links

Gene Symbol

STAT3

UniProt

P40763

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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