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mTOR Antibody

SKU: orb2651437

Description

Rabbit polyclonal antibody to mTOR

Images & Validation

Tested ApplicationsIF, WB
Dilution RangeWB: WB (1/500 - 1/1000), IF/IC (1/100 - 1/500), IF: WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)
ReactivityHuman, Mouse, Rat, Virus

Key Properties

HostRabbit
ClonalityPolyclonal
Clone No.MTOR
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

FRAP; FRAP1; FRAP2; RAFT1; RAPT1; Serine/threonine-protein kinase mTOR; FK506-binding protein 12-rapamycin complex-associated protein 1; FKBP12-rapamycin complex-associated protein; Mammalian target of rapamycin; mTOR; Mechanistic target of rapamycin; Rapamycin and FKBP12 target 1; Rapamycin target protein 1

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mTOR Antibody

Western blot analysis of mTOR expression in HepG2 (A), MCF7 (B), H9C2 (C), AML12 (D) whole cell lysates.

mTOR Antibody

Immunofluorescent analysis of mTOR staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

UniProt Details

No UniProt data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IF
Immunofluorescence
View Protocol

mTOR Antibody (orb2651437)

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Available Sizes

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50 μl
$ 360.00
DispatchUsually dispatched within 5-10 working days
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