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mTOR Antibody
SKU: orb345523
Description
Images & Validation
−Item 1 of 3
| Tested Applications | ELISA, WB |
|---|---|
| Dilution range | ELISA: 1:50,000 - 1:100,000, WB: 1:250 - 1:2,000 |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 2430-2460 of human mTOR. |
| Purity | This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. Reactivity occurs with both phosphorylated and non-phosphorylated forms of mTOR at S2448 from human derived tissues and cells. A BLAST analysis was used to suggest cross reactivity with mTOR protein from rat and mouse based on 100% homology with the immunizing sequence. Expect partial reactivity against mTOR homologues from zebrafish (94%) and dog (89%). Reactivity against homologues from other sources is not known. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
|---|---|
| Form/Appearance | Liquid (sterile filtered) |
| Buffer/Preservatives | Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Concentration | 0.9 mg/ml |
| Dry Ice Shipping | Please note: This product requires shipment on dry ice. A dry ice surcharge will apply. |
| Disclaimer | For research use only |
Alternative Names
−rabbit anti-mTOR pS2448 antibody, FKBP12 rapamycin complex associated protein antibody, Serine/threonine-protein kinase mTOR, FK506-binding protein 12-rapamycin complex-associated protein 1, Mammalian target of rapamycin, mTOR, Mechanistic target of rapamycin, Rapamycin and FKBP12 target 1, Rapamycin target protein 1, FRAP, FRAP1, FRAP2, RAFT1, RAPT1
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Diagram of Metabolic and autocrine regulation of the mTOR pathway by b-cells.
ELISA results of purified Rabbit anti-mTOR Antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1 µg of conjugate. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) (p/n orb347654) and TMB ELISA Peroxidase Substrate (p/n orb348651).
Western blot using Biorbyt's Affinity Purified anti-mTOR antibody shows detection of a band ~245 kDa corresponding to human mTor (arrowhead). Approximately 30 µg of HEK293 cell lysate (p/n orb348669) was separated by 4-8% SDS-PAGE and transferred onto nitrocellulose. After blocking, the membrane was probed with the primary antibody diluted to 1:650 for 2h at RT. The membrane was washed and reacted with a 1:10000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature.
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WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
mTOR Antibody (orb345523)
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