Recombinant Spectrin beta III Antibody / SPTBN2

SKU: orb639749

Description

Spectrin is an actin binding protein that is a major component of the plasma membrane skeleton. Spectrins function as membrane organizers and stabilizers by forming dimers, tetramers and higher polymers. Vertebrate spectrins have two alpha-subunits (alpha-I/alpha-II) four beta-subunits (beta-I-beta-IV) and a beta-H subunit creating diversity and specialization of function. Spectrin and spectrin are present in erythrocytes, whereas spectrin II (also designated fodrin ) and spectrin I (also designated fodrin ) are present in other somatic cells. The spectrin tetramers in erythrocytes act as barriers to lateral diffusion, but spectrin dimers seem to lack this function. Spectrin III is highly homologous to both spectrin I and spectrin II. Spectrin III is highly expressed in brain, kidney, pancreas and liver, and at lower levels in lung and placenta. Spectrin beta 3 is primarily expressed in nervous tissues with highest expression levels in the cerebellum, where it is found in Purkinje cell soma and dendrites.

Research Area

Epigenetics, Neuroscience

Images & Validation

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Tested Applications	ELISA, WB
Dilution Range	ELISA (order BSA-free format for coating),Western blot: 1-2ug/ml
Reactivity	Human
Application Notes	Optimal dilution of the recombinant Spectrin beta III antibody should be determined by the researcher.

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Recombinant
Isotype	Mouse IgG1, kappa
Clone No.	rSPTBN2/1778
Immunogen	A recombinant human partial protein (amino acids 356-475) was used as the immunogen for the recombinant Spectrin beta III antibody.
Purification	Protein G affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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Recombinant Spectrin beta III Antibody / SPTBN2

Western blot testing of human HeLa cell lysate with recombinant Spectrin beta III antibody. Predicted molecular weight ~246 kDa.

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using recombinant Spectrin beta III antibody (clone rSPTBN2/1778). These results demonstrate the foremost specificity of the rSPTBN2/1778 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free recombinant Spectrin beta III antibody (clone rSPTBN2/1778) as confirmation of integrity and purity.

Quick Database Links

UniProt

O15020

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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