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Featured
Description
Research Area
Cell Biology
Images & Validation
−Item 1 of 4
| Tested Applications | IF, IHC, WB |
|---|---|
| Dilution Range | WB: 1-500-1-1000, IF/ICC: 1-100-1-500 |
| Reactivity | Human, Mouse, Rat, Sheep |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human mTOR. The exact sequence is proprietary. |
| Target | MTOR |
| Molecular Weight | 288 kDa |
| Purification | The antibody was purified by immunogen affinity chromatography. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−FRAP; FRAP1; FRAP2; RAFT1; RAPT1; Serine/threonine-protein kinase mTOR; FK506-binding protein 12-rapamycin complex-associated protein 1; FKBP12-rapamycin complex-associated protein; Mammalian target of rapamycin; mTOR; Mechanistic target of rapamycin; Rapamycin and FKBP12 target 1; Rapamycin target protein 1
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Immunofluorescent analysis of mTOR staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Immunohistochemical analysis of paraffin-embedded human breast cancer using mTOR Rabbit Polyclonal Antibody orb213950 with dilution at 1:100.
Immunohistochemical analysis of paraffin-embedded rat kidney using mTOR Rabbit Polyclonal Antibody orb213950 with dilution at 1:100.
Immunohistochemical analysis of paraffin-embedded rat stomach using mTOR Rabbit Polyclonal Antibody orb213950 with dilution at 1:100.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
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Nan Huang 1, Junxi Ren 1, Xinyue Deng 1, Qize Bao 1, Genjie Huang 1, Shimeng Zhi 1 2, Yuedan Li 1, Juan Li 1, Binghui Hu 1, Dongqiang Zeng 1, Huiying Sun 1, Wei Zeng 3, Min Shi 1, Wangjun Liao 1 3, Jianhua Wu 1, Na Huang 1 Endothelial F3-mediated autolysosome and lipid metabolism promote resistance to anti-VEGFA therapy in metastatic colorectal cancer Autophagy ., (2025)
Applications
WB
Reactivity
Mouse
mTOR Rabbit Polyclonal Antibody (orb213950)
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